Literature summary extracted from
Chen, S.; Tomchick, D.R.; Wolle, D.; Hu, P.; Smith, J.L.; Switzer, R.L.; Zalkin, H.
Mechanism of the synergistic end-product regulation of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase by nucleotides (1997), Biochemistry, 36, 10718-10726.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.4.2.14 |
crystals of the ternary enzyme-ADP-GMP complex are grown in glass melting point capillaries by the microbath method, 20 mg/ml enzyme are incubated with 1 mM ADP, 1 mM GMP, and 5 mM MgCl2 and mixed with an equal volume of 24% polyethylene glycol 8000, 200 mM KCl, 50 mM N-(2-hydroxyethyl)piperazine-N'-3-propanesulfonic acid, pH 7.9, brown crystals emerge after 6-12 weeks |
Bacillus subtilis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.4.2.14 |
ADP |
50% inhibition of wild-type, S283A, K305Q, R307Q and S347A mutant enzyme at 4.7 mM, 24 mM, 31 mM, 28 mM and 8.1 mM respectively |
Bacillus subtilis |
|
2.4.2.14 |
AMP |
50% inhibition of wild-type, S283A, K305Q, R307Q and S347A mutant enzyme at 0.9 mM, 6.1 mM, 2.5 mM, 2.6 mM and 1.5 mM respectively |
Bacillus subtilis |
|
2.4.2.14 |
GMP |
50% inhibition of wild-type, S283A, K305Q, R307Q and S347A mutant enzyme at 9.4 mM, 6.6 mM, 50 mM, 50 mM and 145 mM respectively |
Bacillus subtilis |
|
2.4.2.14 |
IMP |
50% inhibition of wild-type and S347A mutant enzyme at 26 mM and 41 mM respectively |
Bacillus subtilis |
|
2.4.2.14 |
additional information |
strong synergistic inhibition with ADP and GMP |
Bacillus subtilis |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.4.2.14 |
Bacillus subtilis |
P00497 |
- |
- |